Influence of composition of culture medium on organogenesis of Thymus serpyllum L. in vitro

Abstract

Thymus serpyllum L. is a species of a flowering plant in the mint family Lamiaceae. Thymus serpyllum L. is widely used in medicine and pharmaceutics, in cookery, in perfumery, cosmetology, as a honey plant, and as an ornamental plant. As a result of the man’s impact (developing of virgin lands, unregulated recreation, degradation as a result of grazing of cattle and other types of breaking) the area of the creeping thyme in Northern Kazakhstan reduces noticeably. So, an important task is the development of the ways of conservation of this valuable steppe plant. The using of methods of biotechnology is one of the optimum decisions of the given question. Initial explants — nodal segments of Thymus serpyllum L. — were cultivated on culture media Murashige and Skoog (MS), ½MS, modified by Collet of Murashige and Skoog (CMS), modified Nitsch and Nitsch (NN), Schenk and Hildebrandt (SH). Modified NN medium proved to be the most favorable for cultivation of explants of T. serpyllum L.After six weeks of cultivation of the creeping thyme on a given culture medium, an average length of shoots amounted to 7.13±0.9 cm, the frequency of rooting — 92.5 %, an average length of roots– 1.52±0.4 cm. The multiplication factor was equal to 1:9