Preparation and characterization of empty nanoparticles of poly-D,L-lactic acid and serum albumin

Abstract

The methods of preparation of polymeric nanoparticles and nanocapsules on the basis of such polymers as human serum albumin and poly-D,L-lactic acid are shown in this article. Human serum albumin and poly- D,L-lactic acid are well-known polymers used in medicine for different purposes including delivery of some potent drugs in the forms of nanoparticles. Empty nanoparticles of human serum albumin and poly-D,L-lactic acid have been successfully synthesized using desolvation and nanoprecipitation methods correspondingly. Optimal conditions of synthesizing the nanoparticles of human serum albumin and poly-D,L-lactic acid have been worked out. Obtained nanoparticles of the polymers have satisfactory physicochemical characteristics: the particle sizes were in the interval of 100–300 nm with narrow particle size distribution which points on the formation of nanoparticles of similar size. The surface charge of the particles was defined as it is the parameter that determines the stability of the system. The meanings of zetapotential were around –30 mV for both systems which justifies the stability of particles within the time. So the results of this study have shown that synthesized nanoparticles of human serum albumin and poly-D,L-lactic acid have good characteristics and hence they can further be used for loading them with different drugs.