Synthesis of Stabilized Manganese-Containing Nanoparticles with Bombyx mori Chitosan Macromolecules under In Situ Conditions

Abstract

Manganese nanoparticles are highly reactive and tend to aggregate and oxidize, which limits their practical application. Therefore, the aim of this study was to synthesize and stabilize Mn nanoparticles using Bombyx mori chitosan as a natural biopolymer matrix under controlled in situ conditions and to investigate their structural and physicochemical properties. Samples containing Mn nanoparticles stabilized with chitosan in the presence of reducing agents, with hydrodynamic dimensions of 118 nm (97 %) and 144 nm (96 %), were synthesized under in situ conditions. The structural and morphological characteristics were studied using IR spectroscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), and electron microscopy. IR spectroscopic studies revealed local rearrangements within the repeating units of the chitosan polymer chain and interactions between the –NH and –C=O functional groups and manganese-containing nanoparticles. XRD analysis confirmed the reduction of Mn2+ ions. Morphological studies of chitosan–manganese films showed the formation of 50 nm tetragonal and 90 nm spherical metal nanoparticles under the selected synthesis conditions. The results confirm the stabilization of Mn nanoparticles with Bombyx mori chitosan by the chemisorption method and demonstrate effective control of particle size and morphology. Further research should focus on evaluating long-term stability, functional properties, and potential biomedical applications of the synthesized nanocomposites.