Indirect method of histochemical assessment of insulin content in pancreatic β-cells

Abstract

Methods for analysis the state of histostructure and insulin and zinc content in pancreatic tissue allow to estimate either a state of histostructure alone or visual insulin content in ß-cells. Zinc content indicating the content of deposited insulin and the ability of ß-cells to form this form of hormone is possible using an additional method that increases the volume of work and complicates investigation. Authors have proposed a method that allows to simultaneously evaluate both the state of the histostructure and the quantitative content of deposited insulin and zinc in ß-cells of the pancreas. It has been experimentally confirmed that both the aldehyde-fucshsin method of insulin staining and Dithizone method for staining of zinc-ions in ß-cells can be separately used simultaneously for quantitative analysis of the content of deposited insulin and zinc in ß-cells. It has been experimentally confirmed that both the aldehyde-fucshsin method of insulin staining and the Dithizone method of detecting zinc in ß-cells can be separately used simultaneously for quantitative analysis of the content of deposited insulin and zinc in ß-cells. The use of both methods allows not only to assess the state of the histostructure, but to assess the content and functional ability of ß-cells to synthesize insulin, as well as to form its deposited form using zinc.