Selection of an effective DNA extraction method for the detection of Salmonella by the polymerase chain reaction

Abstract

DNA extraction is a crucial step at using molecular and biological methods, since the result of PCR analysis largely depends on the quality and quantity of the obtained DNA preparations. In this work, we compared the efficiency of DNA extraction from bacteria in various ways: the trizol method using TRizol commercial reagent, the CTAB method and the sorbent method using silica particles. It has been shown that the extraction of DNA from Salmonella bacteria on silica particles ensures higher sensitivity than the other methods. The amplification of the extracted DNA on silica particles is more efficient and allows obtaining reproducible results with the accuracy of a high degree. In the future, this method will be used to complete the test system for diagnosing salmonellosis. Introduction of the methods of express diagnosis into practice is extremely important, as quick and early diagnosis will allow counteracting the spread of infection in a timely manner, which in turn will improve the epidemiological and epizootological situation in the country.