High specific fluorescent method staining of zinc-insulin complex in pancreatic B-cells

Abstract

Authors demonstrated results using of high specific fluorescent method histochemical staining of Zn+2-ions in pancreatic B-cells. It was showed in diabetic animals and in animals past mobilization of insulin from B-cells a simultaneous decreasing as of amount of insulin as of Zn+2-ions in cytoplasm of B-cells. Meanwhile for- mation in B-cells of chemical complexes of derivatives of Diethyldithiocarbamic acid with Zn+2-ions result negative fluorescent reaction for Zn+2-ions but positive reaction for insulin using of insulin staining methods.