Development and validation evaluation of methods of quantative determination of pulegone in the Ziziphora bunge essential oil

Abstract

Validation characteristics of analytical methods of quantifying pulegone in the essential oil of herb Ziziphora bungeana Juz have been studied. The results of validation showed that the above analytical technique meets the requirements of the test for specificity, accuracy, is characterized by a linear dependence in the investigated range of application of analytical techniques, proper accuracy and convergence of the results. A regulated rate of the main component pulegone in the essential oil of Ziziphora bungeana Juz has been de- termined: its contents must be not less than 40 %.