Antiradical activity and bioprediction of o- and p-hydroxybenzoic acid hydrazide derivatives

Abstract

This article provides results of evaluation of synthesized biologically active substances 1–4 antiradical activity according to their ability to interact with the 2,2- diphenyl-1-picrylhydrazyl radical (DPPH•). It was found that all synthesized compounds 1–4 have antiradical activity. Pronounced antiradical activity was shown by N-ethyl-2-(2-hydroxybenzoyl)hydrazine-carbothioamide (1) (IC50(DPPH) = 12.5 μМ) and N-ethyl-2-(4-hydroxy-benzoyl)hydrazine-carbothioamide (2) (IC50(DPPH) = 16.7 μМ) samples under the conditions of this test sys-tem. Activity of N-ethyl-2-(2-hydroxybenzoyl) hydrazinecarbothioamide (1) and N-ethyl-2-(4-hydroxyben-zoyl)hydrazinecarbothioamide (2) samples are comparable to activity of known antioxidants. Studied sub-stances 1 and 2 are promising for further advanced research of their antioxidant properties and other types of biological activity. Results of PASS computer system applicability estimation for prediction of biological ac-tivity using structural formula as a part of pre-experimental screening are provided. Expected activity of chem-ical compounds combines physiological activity of initial hydrazides of o- and p-hydroxybenzoic acids as well as components of structural molecule. According to bioprediction data analysis, all compounds can act as in-hibitors with a high degree of probability.