Preparation, characterization and investigation of in vitro release of anti-tuberculosis drug p-amino salicylic acid based on human serum albumin

Abstract

Nowadays the task of pharmaceutical chemistry is not only the search of the new drug preparations, but also the increase of the effectiveness of the latter by chemical modification or creation of new forms. With the aim of prolongation of the effect and decrease of single dose the possibility of immobilization of anti-tuberculosis drug p-amino salicylic acid (PASA) on human serum albumin (HSA) nanoparticles (NPs) by adsorption and incorporation methods was considered in this article. It is shown that independently of the immobilization method of the drug into polymer matrix the meanings of binding degree are very high. The study of the dependence of binding degree on drug concentration resulted in more than 95 % at maximum concentrations of the added drug. As it has been expected the drug release degrees have high meanings when incorporating as well as adsorption the drug on the surface of empty nanoparticles. They are 70 % and 80 % respectively. Therefore the results obtained allow us to hope on prolongation of the effect of p-amino salicylic acid and decrease of single dose of the drug in tuberculosis therapy.