Victoria-4 histochemical method staining of insulin

Abstract

Results using of histochemical Victoria-4 method staining of insulin in pancreatic B-cells are presented by authors comparatively with results of using other histochemical methods:immunofluorescent, immunohistochemical, pseudoisocyanine and aldehyde fucshine. By authors it is shown that this method being specific concerning insulin revealing in B-cells, is not high precise for quantitative measuring of insulin content in cells because not only B-cells but blood vessels and connecting tissue of islets are painted in dark colour registered by a photometer as well as blue colour of B-cells. Using of Victoria-4 method is possible for stain of various histostructures of islets that gives possibility to estimate the state of histostructure of islets not only insulin content.