Histochemical and immunocytochemical investigation of endocrine tissue of pancreas after administration of B-cytotoxic chemicals

Abstract

Authors showed that intravenous injection amino acids Cystein and L-Hystidine prevent in majority of animals developing of experimental diabetes caused by chemicals formed in B-cells chelat complexes with Zn+2-ions. It was shown that in all cases administration of these amino acids prior injection of diabetogenic chelator as Diphe nylthiocarbazon (Dithizon) accompanied by almost complete absence of binding of Zn+2-ions in В-cells with formation of toxic complexes which result destruction and death of B-cells within short time. Authors suppose that protective effect is determined by presence in molecules of Cystein and L-Hystidine of SH-groups reacted with reacted with Zn+2-ions in B-cells with forming of not toxic complexes and not able to forming of complexes with a Dithizon. Authors showed also that from the used various histochemical and immunocytohistochemical methods insulin staining in B-cell more precise results was obtained using of immunohistochemical and fluorescent Diethylpseudoisocyanine methods. However, Aldehyde- fuchsine method and method Victoria 4R more suitable for investigation state of histostructure of pancreatic islets.