Histochemical method for fluorescent staining of Zn+2-ions in glands

Abstract

It is showed by authors that using of 8-para(toluenesulphonylamino)quinoline — a derivative of 8-oxyquinolin result histochemical revealing by using of fluorescent microscopy of Zn+2-ions in cells of tissue of prostate, in pancreatic B-cells and in salivary glands contains a large amounts of ions. This method is high sensitive and high specific for revealing of Zn+2-ions and there are only one step of staining procedures. Meanwhile it is possible to use only fresh frozen sections of tissues for investigation within short time limited by 15–20 min.