About quantitative determination of flavonoids in vegetative raw materials

Abstract

Numerous researches show that the medicines created on the basis of flavonoids are high performance antineoplastic tools, have antioxidatic properties, reduce risk of diseases of cardiovascular system. Currently physical and chemical methods of the analysis, such as spectrophotometry, absorption spectroscopy are widely used for the identification and quantitative determination of flavonoids in medicines. However, combined methods involving various variants of chromatographic separation of the investigated components are increasingly spreading. Wide use of physicochemical and combined methods of analysis is primarily due to the fact that these methods have a much greater sensitivity and selectivity in comparison with modern chemical and electrochemical methods. Literary data on researching new, and also development and exploitation of existing methods for quantitive determination of flavonoids in vegetative raw were generalized and analyzed. Special attention is paid to such analyzing methods as method of high-performance liquid chromatography, spectrophotometry and chromato-mass-spectrometry. The HPLC method is a fast, highly reproducible method that requires a small amount of analyte and is used for quantitative, qualitative analysis and preparative release. The most accessible and objective method for controlling biologically active compounds in plant raw materials is the chromato-mass-spectrometric method of analysis, which makes it possible to identify individual flavonoids, regardless of the presence of extraneous or related compounds. It is established that most of the studies are based on the methods for the quantitative determination of flavonoids, set out in the European and British pharmacopoeias, and are widely used to standardize many types of raw materials containing flavonoids.